Euroflow

EuroFlow BCP-ALL MRD 2026

Resources

Detailed information

EuroFlow SOPs and relevant documents

  • EuroFlow SOP for bulk lysis in MRD panels. Version 2.0 – June 2025

  • EuroFlow SOP for instrument setup and compensation for DxFlex. Version 2.0 – February 2025

  • EuroFlow SOP for instrument set-up and compensation for BD LSR II and BD FACS Canto II. Version 2.0 – February 2025

  • EuroFlow SOP for instrument set-up and compensation for BD Lyric for 8-color panels. Version 2.0 – June 2025

  • EuroFlow SOP for instrument set-up and compensation for BD LSRFortessa X-20. Version 2.3 – February 2025

  • EuroFlow SOP for instrument set-up and compensation for Navios. Version 1.5.1 – October 2019

  • Composition and technical info of EuroFlow panels for hematological malignancies. Version 1.12 – February 2025

  • List of reference and alternative antibodies for hematological malignancies panels. Version 1.14 – February 2025

  • Uniform coding of EuroFlow panels. Version 1.1 - March 2019

 Downloads for BD Lyric (if applicable):

  • Reagent files (zip) (download and extract) – June 2025

  • Assay files (zip) (download and extract) – June 2025

  • Label file (xml)

The above documents can be accessed after creating a EuroFlow account and upon login at https://app.euroflow.org/login. 

Videos

Educational video: EuroFlow SOP for Bulk Lysis

euroflow.org

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Educational video: EuroFlow SOP for instrument setup and compensation (for BD LSR II and BD FACS Canto)

euroflow.org

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Publications

Theunissen et al., Standardized flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic leukemia. Blood. 2017 Jan 19;129(3):347-357.

pubmed.ncbi.nlm.nih.gov

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Kalina et al., Quality assessment program for EuroFlow protocols: summary results of four-year (2010-2013) quality assurance rounds. Cytometry A. 2015 Feb;87(2):145-56.

pubmed.ncbi.nlm.nih.gov

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Kalina et al., Frequent issues and lessons learned from EuroFlow QA. J Immunol Methods. 2019 Dec;475:112520.

pubmed.ncbi.nlm.nih.gov

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Nováková et al., How to make usage of the standardized EuroFlow 8-color protocols possible for instruments of different manufacturers. J Immunol Methods. 2019 Dec;475:112388.

pubmed.ncbi.nlm.nih.gov

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Glier et al., Comments on EuroFlow standard operating procedures for instrument setup and compensation for BD FACS Canto II, Navios and BD FACS Lyric instruments. J Immunol Methods. 2019 Dec;475:112680.

pubmed.ncbi.nlm.nih.gov

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